The solubility of KOH protein can reflect the denaturation of protein. Different protein varieties have different solubility. The larger the denaturation of protein, the smaller the solubility of protein. Next, I will explain how to determine its solubility in detail.

1、用一定濃度的氫氧化鉀溶液提取試樣中的可溶性蛋白質(zhì)，在催化劑作用下用濃硫酸將提取液中可溶性蛋白質(zhì)的氮轉(zhuǎn)化為硫酸銨。加入強(qiáng)堿進(jìn)行蒸餾使氨逸出，用硼酸吸收后，再用鹽酸滴定測出試樣中可溶性蛋白質(zhì)含量；同時(shí)，測定原始試樣中粗蛋白質(zhì)含量，計(jì)算出試樣的蛋白溶解度。

1. A certain concentration of potassium hydroxide solution was used to extract the soluble protein in the sample. Under the action of catalyst, concentrated sulfuric acid was used to convert the nitrogen of the soluble protein in the extract into ammonium sulfate. Add strong base for distillation to make ammonia escape, absorb with boric acid, and then titrate with hydrochloric acid to measure the soluble protein content in the sample; at the same time, measure the crude protein content in the original sample, and calculate the protein solubility of the sample.

2、實(shí)驗(yàn)試劑

2. Experimental reagent

1）氫氧化鉀（分析純），無水硫酸鉀、五水硫酸銅、氫氧化鈉、硼酸、甲基紅、溴甲酚綠、硫酸銨；

1) Potassium hydroxide (analytical pure), anhydrous potassium sulfate, copper sulfate pentahydrate, sodium hydroxide, boric acid, methyl red, bromocresol green, ammonium sulfate;

2）濃硫酸、鹽酸（分析純）、百分之九十五乙醇、蒸餾水。

2) Concentrated sulfuric acid, hydrochloric acid (analytical pure), 95% ethanol, distilled water.

3、儀器和設(shè)備

3. Instruments and equipment

1）感量為0.0001g分析天平；

1) The sensitivity is 0.0001g analytical balance;

2）磁力攪拌器；

2) Magnetic stirrer;

3）離心機(jī)（帶離心管），轉(zhuǎn)速為2700r/min以上；

3) Centrifuge (with centrifuge tube), rotating speed is more than 2700r / min;

4）樣品粉碎機(jī)；

4) Sample crusher;

5）60目分析篩；

5) 60 mesh analysis screen;

6）電爐。

6) Electric furnace.

通過以上文中講解的內(nèi)容，大家可以了解到這種氫氧化鉀蛋白質(zhì)溶液的溶解度是如何測定的了。那么，大家在以后的操作使用中，不妨采取這種方法進(jìn)行測定，更加方便、有效。

Through the content explained above, you can understand how the solubility of this potassium hydroxide protein solution is determined. Then, in the future operation and use, we might as well adopt this method for determination, which is more convenient and effective.